Sentence examples for layers of buffer from inspiring English sources

For membrane flotation the mitochondria were treated with Na2CO3 and placed under two layers of buffer containing 0.25 M and 1.4 M sucrose in 20 mM MOPS, pH7.4, and centrifuged for 3 hr at 120,000× g at 4°C.

As the layer of buffer becomes thinner and thinner, however, the air water interface must eventually touch the particle, as shown in Fig. 5B.

In the ideal case, like that shown schematically in A, particles bound to a structure-friendly support film (slide) are not at risk of becoming denatured at the air water interface as long as the surrounding layer of buffer remains thicker than the size of the particle.

In the ideal case, like that shown schematically in Fig. 5A, particles bound to a structure-friendly support film (slide) are not at risk of becoming denatured at the air water interface as long as the surrounding layer of buffer remains thicker than the size of the particle.

As the layer of buffer becomes thinner and thinner, however, the air water interface must eventually touch the particle, as shown in B. Unfortunately, there currently are not known methods to reliably control the sample thickness once it is in the range desired for cryo-EM.

For cryo-EM, samples were vitrified in a thin layer of buffer on continuous carbon support films.

IBM's 3D technique could be used to create a separate circuit layer of "buffers" that would marshall the information flowing to and from a processor, so that designers could create faster processors knowing that they could be kept busy.Three-dimensional stacking offers other potential advantages.

The VG film on the glass substrate is composed of two layers of a horizontal buffer layer and VG nanosheets on that buffer layer.

Currently used designs avoid this well-documented effect by placing a layer of aqueous buffer between polymeric membrane and ISFET serving as internal reference element.

Following incubation, samples were placed onto a layer of Cushion Buffer (80 mM PIPES, pH 7.0, 2 mM MgCl2, 1 mM EGTA, and 60% glycerol) and centrifuged at 100,000  g for 40 min.

The next morning, the medium was replaced with a thin layer of PBS buffer and the cells were irradiated with UV (40 mJ cm−2) using UV Stratalinker 2400 (Stratagene Company, Oceanside, CA, USA).