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This study focuses on the development of a layered construct for osteochondral repair, fabricated through a novel "iterative layering" freeze-drying technique.
Using casting, moulding, freezing and lyophilization techniques, a triple layered construct was prepared consisting of an inner layer of elastin fibres, a middle (porous) film layer of collagen fibrils and an outer scaffold layer of collagen fibrils.
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While there was no significant difference upon addition of the different layers on the elastic modulus (p >.05), the viscous modulus of the single layer construct was significantly lower than the double and triple layer constructs (p <.05).
Furthermore, the cellularized single-layer construct had a relatively high compliance, but the double and triple layer constructs had compliance values comparable to both engineered vessels and native vessels.
Here we present fibroin as a dual-layer construct containing both an epithelium and underlying stroma for corneolimbal reconstruction.
Additionally, when myoblast sheets and HUVECs were alternately sandwiched, endothelial cell connections through the layers and capillary-like structures were found in a five-layer construct.
In this study we created a flexible 3D multiple layer construct that can promote rapid vascularization by this method, and the in vivo results confirmed that there were a large number of blood vessels which grew into the constructs and functionally anastomosed with the in vitro preformed human derived capillaries.
It consists of a two-layered construct with a layer of neonatal karitinocytes seeded on a second layer of collagen matrix containing neonatal fibroblasts (Fishman et al., 2013).
After another 15 minutes polymerization period keratinocytes (0.6 1 × 10 cells/clot) were added on top of the two-layered construct for the epidermal layer.
Keratinocytes were added on top of the two-layered construct to form the epidermal layer.
The double-layered construct was prepared according to the design specifications.
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