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The dried powder was placed onto a glass slide with a thin layer of vacuum grease (Dow Corning High Vacuum Silicone Grease, Sigma Aldrich, St . Louis MO).
The samples were rinsed vigorously with water for 5 minutes, and adhered to the bottom of 60 mm Petri dishes (Becton Dickinson) by applying a thin layer of vacuum grease (Dow Corning) around the perimeter of the unmodified side of the coverslip.
Adult female flies were anesthetized and immobilized on 25-mm glass coverslips with a thin layer of vacuum grease ventral-side-up.
Prior to the experiment, a very thin layer of vacuum grease was spread on the connection surface of the receptor and donor cells to avoid leakage of the samples.
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An additional layer of high vacuum silicon grease was smeared on the rubber discs in order to provide a smooth and frictionless sliding on the end platens.
A thin layer of high vacuum grease (Dow Corning) was applied on the surface of glass bottom by a painting brush prior to placing embryos and larvae in order to minimize their movement during the imaging.
Three- to 5-day-old adults of the appropriate genotype from each cross (in some experiments following thorax measurement) were placed on a slide containing a thin layer of silicone vacuum grease (Beckman, Fullerton, CA) and mounted in Halocarbon 700 oil under a coverslip supported by capillary tubes.
The samples were sealed in the chamber by a 170 microns thick flat sheet of borosilicate glass with the seal being provided by a layer of high vacuum silicone grease between the plastic and the coverslip, then oversealed with DPX mounting medium.
Fig. 12 Schematic illustration of perovskite solar cells fabricated by sequential layer-by-layer vacuum deposition.
ToF-SIMS is a powerful surface analysis technique that allows chemical characterization of the first atomic layers of any vacuum stable solid surface.
For the thin layer of silver in vacuum we see the bright band corresponding to the characteristic surface plasmon polariton dispersion.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com