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When analyzed for alignment, 81% of RGCs were observed to project axons radially along the scaffold fibers, with no difference in alignment compared to the nerve fiber layer of retinal explants.
Those discs at the other end of the stack are 'swallowed' by a single layer of retinal pigment epithelial (RPE) cells.
To determine the extent of glial soma swelling, the cross-sectional area of Mitotracker Orange-stained cell bodies in the inner nuclear layer of retinal slices was measured off-line using the image analysis software of the LSM.
To determine volume changes of Müller glial cells evoked by hypotonic stress, the cross-sectional area of glial cell somata in the inner nuclear layer of retinal slices was measured.
For these conditions the AOSLO was focused at the RNFL and thus the montage shows only the superficial layer of retinal capillaries.
TUNEL+ nuclei in the photoreceptor cell layer of retinal explants cultured without Mos (Fig 6A), with C57BL/6 Mos (Fig 6B), and with Cx3cr1 −/− Mos (Fig 6C) were more numerous in the presence of Mos and particularly in Cx3cr1 −/− Mos.
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In others, regions of thick layers of retinal pigment epithelium (RPE) cells were observed, with RPE cells appearing inside the NR layers in direct contact with the lens (Fig. 7E).
The layers of retinal structures were segmented with an automated segmentation algorithm [ 3].
4′,6-Diamidino-2-phenylindole (DAPI) staining of nuclei in retinal sections revealed no difference in the structure or thickness of the well-defined layers of retinal neurons following TRID treatment (Fig 5A).
All of this could have been avoided or minimized were the rods and cones placed in the most superficial layer of the retinal, rather than buried at the bottom.
New vessels sprout from the deep layer of the retinal vasculature, invade the ONL and subretinal space and eventually these neovascular lesions are engulfed by RPE cells.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com