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Moreover, immunohistochemistry staining in prostate cancer tissues showed the localization of Gal-3+ cells in the basal layer of prostate ducts, confirming the proposal of Gal-3 as a new basal cell marker.
Experiments in our previous study showed that the positive staining of Gal-3 in the luminal layer of prostate epithelium was heterogeneous but uniform in the basal layer, consistent with reports of Ellerhorst et al., suggesting that the expression of Gal-3 might differentially reflect the heterogeneity of prostate cancer.
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Multiple lines of evidence support the hypothesis that stem cells within the basal layer of prostates in noncastrated and castrated animals are responsible for tubule-forming capacity and give rise to multipotent progenitor cells within the basal layer.
In addition, the expression of C/EBPα in the actively proliferating basal cell layer of the prostate suggests that C/EBPα does not cause growth arrest in normal prostate epithelium.
CK19 and Sca-1 positive cells are present both in the luminal and basal epithelial cell layer of the prostate [35], [36], but the expression of other epithelial cell markers differs in these cell populations.
The inner layer of the prostate capsule is composed of smooth muscle with an outer layer covering of collagen [ 7].
The immunohistochemistry findings in Figure 1 indicated that C/EBPα was present in the in the basal layer of normal prostate epithelium while AR and PSA were localized to the differentiated secretory epithelial layers.
The immunohistochemical result demonstrating C/EBPα expression in the basal layer of the prostate epithelium with no expression of PSA in these cells led us to examine the effect of C/EBPα on the expression of the PSA promoter/enhancer.
pAkt overexpression in Clu+Tacstd2+ cells in the luminal epithelial cell layer of normal prostates was never observed (Figure 4E 4F).
Importantly, we also identified at low frequency novel lineage-specific progenitor cells in the luminal epithelial cell layer of normal prostates.
To estimate the number of progenitor cells in the luminal epithelial cell layer of normal prostates at 4 5w and at 4 5m, slices of the mouse anterior lobe were stained for Clu.
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