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Maximal-intensity projections encompassing a single layer of nuclei were generated by the Slidebook software.
Regarding the SVZ, at E40, we observed a slender layer of nuclei basal to the VZ containing nuclei immunoreactive for the BP marker Tbr2 (Fig. 1 B ).
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In the hagfish and those vertebrates in which the oocyte receives heavy deposits of yolk (elasmobranchs, reptiles, birds, and monotremes), the epithelium appears to be two cells thick, apparently the result of layering of nuclei in a simple columnar epithelium (i.e., epithelium consisting of relatively "tall" cells).
Z-series analysis through the high density regions revealed several layers of nuclei (3 4), with mitotic nuclei always in the layer nearest the cuticle ( Fig. 5 ).
The OSVZ was defined as a layer consisting of nuclei more radially aligned than the ones found in the ISVZ and was also distinguishable by a different nuclear density as compared with the ISVZ.
Embryos were scored as gastrulation defective if Z2/ Z3 were found to remain among the external layer of embryonic nuclei (Powell-Coffman et al. 1996).
The posterior region of the embryo with the most ectopic CP110 foci included the PGCs as well as the underlying layer of somatic nuclei, but it was only the PGCs for which obvious changes in cell behavior were detected.
The procedure can detect a too-thin layer of one nucleus depth, when 2D-SSA should be used instead of 3D version.
Its retina is also very similar to ours, with three distinct layers of cell nuclei, comprising the cell bodies of photoreceptors, interneurons, and output neurons (ganglion cells).
The subplate was identifiable within the intermediate zone as a layer of large round nuclei, deep to the cortical plate.
The majority of wild-type airways consisted of an open lumen surrounded by a single layer of uniformly aligned nuclei (Fig. 1J and M).
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