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H22 tumors were not hormone dependent and easily grown in the subcutaneous layer of mice.
The treatment of IFN-α/β increased the antigen-uptake rate of resident APCs in nasal mucus layer of mice (Bracci et al., 2005).
TEM of capillaries in the ganglion cell layer of mice expressing Ad-null revealed normal ultrastructure, with close apposition between ECs and pericytes, a defined extracellular space, and few vacuoles (Figure 7A).
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The inner cell mass – which harbors stem cells – is then carefully extracted and placed on a feeder layer of mouse embryonic fibroblasts that provide nourishment and hopefully prevent the ES cells from differentiating (i.e., converting to a specialized cell).
Conventionally, embryonic stem (ES) cells are cultured on a cell layer of mouse embryonic fibroblasts (MEFs) as feeder cells to support undifferentiated growth of ES cells.
But growing stem cells without a layer of mouse feeder cells is hardly a trump card for the firm.
One such hope: During the fall, Geron was the first to figure out how to grow embryonic stem cells without a layer of mouse "feeder" cells.
He extracted blastocysts from days-old human embryos and grew them on a layer of mouse "feeder" cells in a lab dish.
Mouse ES cells are typically maintained on a feeder layer of mouse embryonic fibroblasts (MEFs).
hES cells were grown on a feeder layer of mouse embryonic fibroblasts (MEF) at passages 1 to 4. MEF were irradiated and seeded at 1.88×105 cells per well in a 6-well plate.
In the culture of human keratinocytes this information has been used routinely since the initial publications by Rheinwald and Green in 1975 [4] using an irradiated feeder layer of mouse fibroblasts (i3T3) to support the attachment and proliferation of adult human keratinocytes in the presence of a mitogens rich media containing 10% bovine foetal calf serum (FCS).
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