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After sonication, the chloroform layer extract was spotted onto a thin layer chromatography plate (GF254, 0.25-mM thickness, 10 × 10 cm).
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Then, for each object proposal that is generated with the saliency algorithm a RoI pooling layer extracts a fixed-length feature vector from the conv feature map.
Then, for each object proposal a region of interest pooling layer extracts a fixed-length feature vector from the conv feature map.
To build soil protection map, we use land use/land cover layer extracted from LANDSAT 7 ETM + images in addition to vegetation cover layer derived from MODIS NDVI product.
After cooling the reaction mixture was added to CH2Cl2/water (1:1, 200 mL), the layers separated and the aqueous layer extracted with CH2Cl2 (3 × 50 mL).
The reaction was then diluted with EtOAc (50 mL), the layers separated and the organic layer extracted with aq citrate (10%%, 2×50 mL).
The resultant biphasic mixture was separated and the aqueous layer extracted with CH2Cl2 (3×10 mL), the CH2Cl2 layers were then combined, dried (MgSO4), filtered and concentrated in vacuo.
The reaction was then diluted with EtOAc (50 mL), the layers separated and the organic layer extracted with aq HCl (1 m, 3×25 mL).
The residue was redissolved in H2O/EtOAc (160, 160 mL), the layers were separated, and the aqueous layer extracted with EtOAc (80 mL).
After 20 min EtOAc (80 mL) was added and the organic layer extracted with saturated NH4Cl solution (2 × 40 mL) and H2O (3 × 40 mL).
The remaining residue was taken up in CH2Cl2 (100 mL) and washed with 10%Na2CO33 solution (80 mL) and the aqueous layer extracted with CH2Cl2 (60 mL × 3).
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