The phage lysates of titer of 109 plaque forming units (PFU) per ml were diluted 10−2 (i.e. 107 PFU/ml) and 10−4 (i.e. 105 PFU/ml) and applied in triplicate for both the dilutions by spotting 10 µl aliquots onto soft agar lawns inoculated with tested S. aureus strain.
Repeat this entire procedure for each additional garden, landscape bed, and/or lawn area you wanted tested.
In brief, disks containing 30 µg of cefotaxime, ceftazidime or cefepime and disks containing a combination of each of these drugs with 10 µg clavulanic acid (Bio-Rad, Nazareth Eke, Belgium) were placed independently on a 0.5 McFarland opacity lawn culture of the tested isolate on a ISO Sensitest® Agar plate and incubated for 18 24 h at 37°C.
After 1 hr, less than 5% of animals were found outside the bacterial lawn for all strains tested; these animals were not counted.
The virulence of all 98 isolates (one isolate forming a thick bacterial lawn was excluded) was tested in triplicate, using a C. elegans host model, with the strains NCTC8325 and M92 representing positive and negative reference strains, respectively [ 13].
In the D. discoideum phagocytosis model [21], nine dilutions of Dictyostelium cells with 2.5-fold interval, starting from 2.0×105 cells, were spotted onto bacterial lawns formed by the tested P. aeruginosa strains.
39 Recently, paper disks were impregnated with sera from patients recruited to a study of community-acquired pneumonia/bacteremia in NE Thailand and tested against lawns of Staphylococcus aureus 24% of patients had evidence of anti- S. aureus activity in their sera.
Different dilutions of these homogenates containing lambda phage were tested on BB4 lawn and PFU counted.
Between 25 and 30 C. elegans worms were transferred from a lawn of Escherichia coli OP50 to a lawn of the bacterium to be tested, incubated at 25°C, and examined at 24-h intervals with a stereomicroscope (Leica MS5) for viability.
To test whether SMX-treated E. coli encountered by worms had a decreased ability to proliferate, we tested E. coli lawns for cell viability.
