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If you're the former, you may remain; if the latter, kindly leave".
Human 17β-HSDs 2, 4, 5 (AKR1C3, the latter kindly provided by Dr. T. Penning) and 7 were all cloned into the modified pGEX-2T vector.
Sea urchin ADP-ribosyl cyclase sequences were determined by PCR screening of Strongylocentrotus purpuratus cDNA libraries derived from the testes and/or ovary, the latter kindly provided by Professor Gary Wessel, Brown University, USA.
Antibody against M. sexta chitinase 5 (anti-MsCht5, specific for group I chitinases) and anti-sand fly Cht8 sera, the latter kindly provided by Dr. Ramalho-Ortigao (Department of Entomology, Kansas State University), were used for immunostaining of AgCht5 and AgCht8, respectively, in mosquito pupae.
Retention times and UV spectra were compared with those of freshly prepared standard solutions of PCA and FA (Sigma, St . Louis MO), and 5-5-DFA, the latter kindly provided by the laboratory of Dr. J.T. Arnason (University of Ottawa, Ontario, Canada).
For this purpose, Balb/c WT and Balb/c.Cd59a /– mice (the latter kindly provided by Professor Marina Botto, Imperial College, London, UK) were infected with the same low dose of influenza virus used to infect the B6 mice, and weight loss was monitored daily.
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The latter were kindly provided by J. Brüning (Institute of Genetics, Cologne, Germany).
The latter transfectants were kindly provided by Dr Franco Fais, Department of Experimental Medicine, University of Genova, Italy.
Although most transition probabilities remained constant, 'all other cause death', 'post- and peri-operative death' and 'healthy to low-risk adenoma' varied by patient age: the latter probabilities being kindly supplied by Dr Frazier (Frazier, 2002).
While I fell foul of the booking system for the latter, HTC's booth kindly ushered me into one of its darkened rooms and the result was frankly mind blowing.
(The latter two were both kindly donated by Dr A Greiner, University of Würzburg, Germany).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com