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This latter affinity is similar to that reported by another group using ITC and biolayer interferometry (Ordureau et al, 2014).
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ADORA2A and ADORA2B are both adenosine-activated GPCRs, the latter exhibiting lower affinity for this agonist [19].
The latter are high affinity interactions that have been reported to suppress the transcription of several tumor suppressors (e.g., pRb, p16, HIC) through interaction with inappropriately methylated CpG islands [30].
The latter has an affinity for the chelant that is hundreds of times greater than that of Mn2+.
The competition for Bcl-xL was reported between Bax and Bad, with the latter having higher affinity [ 42].
Such a function will be determined by the dissociation rate (Kd) of PABP1 which is influenced by whether it is bound non-co-operatively or co-operatively to poly(A), with the latter exhibiting higher affinity [ 12].
Ucn-1 is a neuropeptide of the corticotropin-releasing factor (CRF) family and binds to CRF receptors 1 and 2, to the latter with higher affinity than CRF itself [ 65].
Unlike the PCA, the Altaian Kazakhs were clearly separated from the indigenous Kazakh populations, with the latter showing greatest affinities with lowland Kyrgyz.
Note that the non-specific binding to the negative control sequence (CRE, cAMP response element) is within this latter range of affinities (Kd = 6.6 µM).
The non-specific binding of peptide HTHi to the negative control sequence, CRE, is within this latter range of affinities; Kd = 6.6 µM. ; comparatively, that of peptide K156 was 16 µM [43].
DNA-binding assays revealed that both the polymerase and ligase domains bind DNA in vitro, the latter with considerably higher affinity.
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