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For duplicate entries, we generally kept the sequence from the highest ranked database, and if there was more that one from this database we kept the latest version of the sequence.
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Genotype analysis is performed using the latest version of the 7900HT Sequence Detection Software (SDS v2.3) and confirmed by manually by the technician.
In order to acquire the up-to-date genomic annotation data for functional enrichment analysis, all CNVR breakpoint coordinates were converted to the latest version of the human reference sequence (from NCBI36/hg18 to GRCh37/hg19) (Supp. Materials and Methods).
The physical coordinates of integrated interval limits on the latest version of the PN40024 reference genome sequence (assembly version 12X.2; [ 123]) were deduced from local recombination rate between flanking SNP markers with known physical coordinates [ 106].
2) non-specific PCR amplification when evaluating the specificity of the primers using MFEprimer [ 11]; 3) cross-RNAi [ 2] discovered using the BLAST program [ 12] to search for the predicted PCR amplicons against the latest version of the C. elegans genomic DNA sequence; 4) mis-operation, such as sample loading error [ 1], etc.
In the latest version of the A. queenslandica genome the LNX1/2 sequence is identified as transcript Aqu1.226425 http://www.metazome.net/amphimedon.net/amphimedon
We used a combined Roche-454 and SOLiD sequencing approach and mapped all sequence reads on the latest version of the Duroc pig genome (Sus scrofa 10.2) which is the only available porcine genome assembly at the chromosome level.
FLAGdb++ also contains TAIR gene annotations, available transcript sequences and the latest version of the Eugene predictions (v1.59) for the Arabidopsis genome.
Primers for amplification and sequencing were designed using the latest version of the human genome assembly (GRCh37; http://www.ncbi.nlm.nih.gov/projects/genome/assembly/grc/index.shtml).shtml
Hornet Windmill is the latest version of the Hornet Software for Project Management.
The latest version of the encyclopedia is 858 pages.
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