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Preliminary experimental comparison and analyses indicate that, the lateral and axial resolutions of PFDCM are better than 0.2 μm and 2 nm, respectively, when wavelength of incidence laser beam λ= 632.8nm, numerical aperture of measuring lens NA= 0.85, and lateral spot size with a three-zone phase-only pupil filter GT= 0.65.
By limiting its aperture, we could demonstrate an effective excitation PSF having a lateral spot size of 209±20 nm in vacuum.
This small lateral spot was observed in the three studied neonatal mice (arrows in Fig. 3).
Second, SERT mRNA expression was also found at a more lateral spot, about 500 µm from the midline, just lateral to the pyramidal tract (py), in an area possibly corresponding to the para-pyramidal subgroup of 5-HT neurons (ppy in Fig. 3).
AP and lateral spot fluoroscopic images are taken to determine 3D nail position and rotation.
Upon completion of the 1-, 2-, 3- and 4-h scans, 5-min right and left lateral spot views of the skull were acquired.
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Echis coloration includes various shades of brown, gray, or orange with darker dorsal blotches and lateral spots.
Abdomen with patterns forming a median strip and dark lateral spots, coxopleural streak present, hind coxa bare on posterior surface…………………Paramacronychiinae Brauer & Bergenstamm.
tend to have reddish-orange dorsal fins (high DRG values) with relatively few dorsal-fin spots, and high numbers of lateral spots; G. hubbsihave yellow-orange dorsal fins (low DRG values) with moderate numbers of dorsal-fin, and lateral spots; G. mannialso has reddish-orange dorsal fins (high DRG values) with large numbers of dorsal-fin spots, and few lateral spots.
These findings were also consistent with the expression of HEC1 in lateral spots at the apical part of the early gynoecium (Fig. 4J).
Examination of canonical loadings revealed that all four traits (DRG, caudal-fin spots, dorsal-fin spots, and lateral spots) were strongly correlated with one or more canonical axes (Table S4).
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