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CPC5 accounts for the cell attaching the lateral protrusion to the substrate (Fig. 3b), whereas in CPC2 the lateral protrusion is not attached (Fig. 3b).
The two asymmetric canonical principal components, CPC2 and CPC5, were found to be associated with lateral protrusion of pseudopods.
The same rationale is applied for the interpretation of a lateral protrusion in the basisphenoid of several chelids and in Araripemys barretoi Price, 1973 [ 102- 104].
The basisphenoid of this species shows a very reduced lateral protrusion just anterior to the foramen posterius canalis carotici interni ([ 100], Figure 2B; [ 101], pl. 1C).
We identified four additional canonical components, reproducible from cell to cell, overall accounting for an additional ~20% of mechanical work, and associated with events such as lateral protrusion of pseudopodia.
The results from this analysis showed that the protrusion of lateral pseudopods takes about 12 s in average, starting with the lateral protrusion leading to the bending of cell shape and finishing with the attachment of the pseudopod to the substrate.
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It is noteworthy that after photoconversion, lateral protrusions (especially noticeable at V > + 2 V) are discerned close to each nonacene molecule (Fig. 3a,b).
Importantly, after applying an alternative, thermal approach wherein the sample is annealed to 160 190 °C rather than exposed to light, lateral protrusions close to nonacene molecules are also observed at certain bias voltages Vbias (see supplementary Fig. 13).
The latter appear in a very early stage of development as lateral protrusions from the wall of the neural tube, which are constricted off from the remainder of the brain rudiment as the optic vesicles.
The long and thin lateral protrusions observed for DPSCs on pSi were formed by actin, confirming them as filopodia.
An alternative, but non exclusive hypothesis is that TIMP-1 could act as a repellent cue for growth cones, subsequently leading to the emergence of lateral protrusions.
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