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The viral supernatant was replaced 24 h later with standard DMEM culture medium containing 2 μg/ml cell culture tested puromycin dihydrochloride (Sigma-Aldrich #5858-2).
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17 Although greater efficacy was observed at later time points with standard IBU formulations compared with IBUNa as measured by PRID, IBUNa provided greater analgesic benefit at earlier time points.
The colors were later replicated in plaster with standard plaster pigments.
Two days later, the medium was replaced with standard differentiation induction medium (α-MEM containing 0.5 mM isobutylmethylxanthine, 1 µM dexamethasone, 5 µg of insulin per ml, 10% FBS, 50 U of penicillin per ml, and 50 µg of streptomycin per ml, and the medium was renewed every other day. The cells were induced for 8 days before analysis.
After 6 h, 1 ml of medium (RPMI 1640 plus 20 % FCS) was added per well; 12 h later, this medium was replaced with standard culture medium (RPMI 1640 plus 10 % FCS).
Later on, the synergistic effects 9 with standard antimalarials {artemisinin (ART) and chloroquine (CQ)} were studied in order to provide the basis for the selection of the best partner drug.
Twenty-four hours later, lentiviral containing media was replaced with standard BV2 culture media for another 48 hours then replaced with 5 mg/ml puromycin in DMEM 10% FBS.
Still later stock-car racing in 1939 started with standard models modified for racing.
Individual BP values were obtained at 4 h postinjection on each of the five mice at both test and retest (8 days later), with the corresponding mean and standard deviation.
In his musical techniques he stands at the summit of the Renaissance, blending traditional forms with innovations that later became standard practices.
The rule does not require compliance until much later, with interim compliance to lesser standards starting in 2022 and final compliance with the full performance standards by 2030.
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