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Later, the expression of hormone/growth receptors (ER, PR, and HER2) was added to the standard stratification of breast cancer patients.
6 days later, the expression of AMPK and p-AMPK were assessed by western blotting and quantitatively analyzed (b).
Twenty-four hours later, the expression of CD19, Cyt1 and Cyt2 was assessed by flow cytometry using CD19-PE (BD Biosciences), or specific monoclonal antibodies against Cyt1 or Cyt2 [29] (in 0.1% saponin).
5 days later, the expression of the neuron marker and the astrocyte marker was detected.
Later, the expression of miR-29 is increased as the matrix matures and the osteoblast achieves terminal maturation.
Eight or 15 weeks later, the expression of p16 and c-myc mRNA was examined by Real-time PCR (Q-PCR).
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However, it has been proven later that the expression of HK genes is still under stringent regulation albeit ubiquitously expressed; their expression levels may vary significantly across different cell types [ 1- 3].
Oct-4 plays a crucial role towards regulating the chromatin structure in a state consistent with self-renewal and later facilitating the expression of genes that keeps the cell poised to respond to cues that lead to differentiation.
However, in the later stages the expression of VvAP2 decreases in male, while in females and hermaphrodites it shows a tendency to increase.
However, in the later stages, the expression of miR-30e* and miR-133b* was dissipated comparing to their counterparts miR-30e and miR-133b, respectively.
We later confirmed the expression of these candidate genes in the mouse RMS using the microarray data of laser microdissected RMS generated by Khodosevich et al. [ 34].
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