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Two days later, the conditioned differentiation medium of C2C12 was replaced by new conditioned differentiation medium from freshly transfected 10T1/2 cells (differentiation condition day 2).
The day later, the conditioned differentiation medium from transfected 10T1/2 cells was added to C2C12 cells (differentiation condition day 0).
Forty eight hours later, the conditioned media was harvested and added to fresh explant cultures at the concentration indicated.
Forty-eight hours later, the conditioned media were analyzed for IL-1Ra and PGE2 concentrations.
Two minutes later the conditioned stimulus and unconditioned stimulus were again administered, and the mice remained in the conditioning chamber for an additional 2 minutes.
Two days later, the conditioned medium was harvested as ASC-CM and UC-MSC-CM.
Similar(53)
Fifteen years later, the conditions were right.
One week later, the conditions were reversed.
He noted, however, that, more than eight months later, the conditions have been improved, and he assured the government that his dismay over the slow response had not colored his decision to free Dr. Lee on bail.
An error which offered all neighbourhood networks a 12-month extension of their current funding - only to be told days later the conditions were being withdrawn and a three-month contract issued instead - was "breathtakingly careless and insensitive", they said.
A little later the conditions, hours, food, and wages of merchant seamen were brought under government regulation; an eight-hour day was set for railway crews; and workmen's compensation laws were instituted.
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