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We defined regions of interest (ROIs) centered on activation peaks predicting Now versus Later selection bias.
A later selection yielded a 2'-fluoropyrimidine-modified aptamer, which offered even greater protection [26].
Forty eight hours later, selection with puromycin (0.7 µg/ml) was started.
This particular preference for eating glutinous foods maybe the effective basis for the later selection of sticky endosperm mutants from normal non-glutinous cereal crops such as rice, foxtail millet, common millet, barley, and maize [32].
The cells were washed twice (2 min) with WB and incubated with the DNA pool on ice in an orbital shaker for 30 min. In later selection rounds, the cells were washed with increased stringency to remove weakly binding sequences (a larger number of washes and increased washing time, up to 5 min).
Cluster diversity generally decreases in later selection rounds, as expected.
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After lucking into Kyrie Irving the year after they lost LeBron James to the Miami Heat, there's been plenty of second guessing about their later selections.
Based on his read of Idzik and of the Jets' situation, Schneider predicted in a recent interview that Idzik would deal a first-round pick in order to stockpile later selections.
Mr. Kahane supported this argument with two sets of his vocal works: first, the "Orinoco Sketches," a formal piece in its first New York performance, and later, selections from his most recent pop disc, "Where Are the Arms" (2nd Story Sound).
Later selections of speeches were reprinted ten and twenty years later, in Letters from a War Zone (1988) and Life and Death (1997).
This selection strategy makes later selections strongly affected by the earlier ones.
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