Sentence examples for later purification from inspiring English sources

The sequence was modified to include a 5′ (Glinker)3 linker sequence to the fused apoptin protein downstream of the anti-CD22 scFv sequence, and a C-terminal 6XHis-tag was added to facilitate the later purification and immunodetection of the fusion protein.

Briefly, the PCR was performed with 10 μM primers, one of which was biotinylated for later purification by Streptavidin Sepharose (VWR).

Cells were then harvested by centrifugation for 20 min at 3,000 g and frozen at -20°C for later purification.

To address these concerns, RNA whole blood collection tubes have been developed that have the considerable advantage of lysing whole blood at the time of collection, while simultaneously stabilizing RNA for later purification.

At this point, the bioreactors were fed with 0.5% serum-containing medium every other day, and spent medium (20 mL) from the ECS was collected and frozen at −20 °C for later purification.

The low coverage of 9% for TBCC is due to the low affinity of TBCC to the TBC-DEG complex, which is successively lost in the later purification steps.

The maximum activity of Mro APO (40,944 U L-1, veratryl alcohol assay) was detected in the presence of 42 g L-1 glucose (300%), 48 g L-1 soybean peptone (700%) and 4.5 g L-1 yeast extract (300%) and later (after purification) found to correlate with roughly 445 mg L-1 peroxygenase protein.

Then the methylated pEGFP-C3 plasmid with enough volume (more than 500 mg) should be prepared and stored in −80 °C after purification for later TDQ experiment.

After purification, 2 μL of assay buffer (in which the samples were dissolved later) were pipetted onto 16 spots on a NanoQuantPlate (TecanGroup) as blank.

Baseline apoptosis was determined immediately after purification.

At 30 minutes post-infection or control, 10ml samples were taken into 1% phenol 19% ethanol (final v/v), incubated at 4°C for 45 minutes, spun down at 3320g for 10 minutes at 4°C before the pellet being stored at −80°C for later RNA purification [2].