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Serial sections (6 µm) were later obtained using a Leitz CM3050 cryostat (Leica Microsystems, Gothenburg, Sweden) and 2 6 sections were placed in each well of a three-well microscope slide (Novakemi, Stockholm, Sweden) pre-coated with 0.1% poly-L-lysine (Sigma, Stockholm, Sweden).
Single base-pair resolution mapping of centromeric chromatin was later obtained using MNase digestion followed by paired-end sequencing of nucleosome-sized particles, which showed that occupancy is precisely delimited to the CDE (Cole et al. 2011).
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Genotypes for several loci (discussed later) were obtained using AcycloPrime-FP chemistry and a Wallac Victor3 plate reader as described in Smith et al. (2005).
Our simulation study given in Figure 6a,b validates the former, while Figure 8a,b replicates the later conclusions which were obtained using theoretical analysis.
Two weeks later, fundus photographs were obtained using a handy fundus camera (Genesis Df; Kowa, Tokyo, Japan).
Twenty-four hours later, fluorescent images were obtained using a confocal laser scanning microscope FV500 (Olympus, Tokyo, Japan), and the percentage of speck-positive cells was calculated as the number of speck-positive cells divided by the total number of transfected cells.
The related statistics were obtained using the later 1.5 × 106 configurations.
BIOFDM was later validated by comparing the simulated results with those obtained using BIOPLUME III for the case study of Shieh and Peralta (2005).
Datasets were obtained using the Stanford Research Institute SRI PathWay tools [32] and by later curating the database with Arabidopsis-rice ortholog annotations.
Comparisons between prediction performances obtained using the P and the K kernels are described later.
Later, using a laser trap system with improved time resolution [ 61], similar results were obtained using fast and slow myosin II from skeletal muscle.
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