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Combination of H-FABP, which is released early, and a later marker such as TnT may reduce the false-negative ratio and provide an optimal diagnostic performance [34].
More significantly, we found that 2BP severely inhibited bone nodule formation, a later marker of osteoblast differentiation and an indicator of osteoblast bone forming activity (Fig. 2D).
However, when a later marker of the endocytic route was used, CD63 characteristic of the phagolysosome, SO2 mutant showed poor colocalization with this marker, displaying a persistence phenotype as described previously by our group for murine macrophages [12].
Given that the mice in our model lacked the inflammatory mediator elevation typical of late-stage NEC, we can extrapolate that the elevation in inflammatory mediators is a later marker of more advanced disease.
The genes analyzed included: α-smooth muscle actin (α-SMA), an early marker of SMC differentiation and highly specific marker for SMCs in adult animals [23]; smooth muscle myosin heavy chain (SM-MHC), a later marker in SMC differentiation that seems to be highly restricted to SMCs [24]; calponin and smooth muscle α-22, definitive SMC markers [25].
Osteoblast differentiation and maturation were evaluated using alkaline phosphatase specific activity as an early marker and osteocalcin secretion as a later marker [ 26].
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However, later markers for neuronal differentiation, like HuC/D and Tuj-1, are not induced by cHes6-1 overexpression (data not shown), suggesting that cells do not progress further into differentiation.
However, expression of later markers of cardiac differentiation were reduced by Xic1Mo.
These were not simply later markers of acute brain injury severity or (for tracheostomy) need for ventilation.
No differences in early mDA differentiation markers, including En-1 and Lmx1A, or later markers, including Th and Pitx3, were detected (Fig. 4A).
We then assayed for expression of neural-β-tubulin, a marker of primary neuron differentiation, and also the later markers of NA differentiation Hand2 and TH (Fig. 3).
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