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Whenever possible, the mites within the samples were isolated immediately; otherwise, they were stored frozen at −20°C for later isolation [ 10, 11].
For here is a man whose toughness has been the source of both his early success and his later isolation.
A part of the liver was kept for later isolation of total RNA.
The sliced samples were then kept in cryo tubes and frozen at -80°C for later isolation of DNA.
At that time, fin clips were collected and stored on Whatman paper for later isolation of DNA.
Remaining tissue was immersed in liquid nitrogen and stored at −80°C for later isolation of total RNA.
Similar(52)
TBP is unstable and extremely light sensitive [24] and contact to air and light was avoided where possible during later isolations from bacterial biofilms.
In other experiments, cells were cryopreserved immediately after isolation and thawed later, as described (Hermann et al., 2007).
THOC5 was mainly detected in nuclear speckle-like domains at an early stage of macrophage culture (4 days after isolation), while at a later stage (8 10 days after isolation), THOC5 was detected diffusely in the nucleus and in the cytoplasm.
Immediately after isolation, the biopsies were placed in RNA later® Solution (Ambion/Applied Biosystems, Foster City, CA).
The spleen was isolated three days later for isolation of lymphocytes and fusion with rat myeloma cell line IR983F [ 43].
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