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Later, in vitro plant axillary buds were tried as explants for transformation, however, transformation frequency was low ranging from 0.5 to 2%.
Importantly, a later in vitro enzyme-substrate study on similar H3-derived peptide substrates has put the impact of PTMs on CATL clipping efficiency into perspective 89.
After dissection, testes were separately stored in 200 μl of a non-activating medium (for mixture see [ 72]) for later in vitro fertilization (sperm from the left testis) and sperm morphology determination (sperm from the right testis).
In a later in vitro study, aqueous crude extracts of qat were demonstrated to possess selective antibacterial activity against periodontal bacteria, with the highest activity against pathogenic species, including Porphyromonas gingivalis and T. forsythia, and least activity against health-compatible ones [ 16].
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Fused cells were found in vivo at the time of sacrifice fifty days later and in vitro after culture of dissociated cells, indicating that they are able to survive in vivo.
Later, the in vitro cytotoxicity of the DOX-loaded PMs against MCF-7 cells was also assessed.
This allowed us to later correlate in vitro islet [Ca2+]i oscillations with in vivo insulin pulsatility in groups of islets taken from these same individual mice.
The same research group later demonstrated in vitro and in vivo that lipid rafts are critical for coupling SOCE to the constitutive activation of PKB/Akt in a Ca2+/calmodulin-, Src-, and PP2A-mediated pathway.
However, this did not really exclude nicotine as a sensory irritant, since it was later demonstrated in vitro and in mice that nicotine in high concentration activates TRPA1 (34).
As the ADI-PEG20-sensitive cell line SK-LC-13 was determined to be tumourigenic, it was chosen to be a model cell line for later experiments in vitro and in vivo.
These phenotypic characteristics were not conserved once the BM26 plants were evaluated eight weeks later in ex vitro conditions (SM and SB).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com