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The biopsy was freed from visible fat and connective tissue, and one piece immediately snap frozen (for later gene expression analyses) and another piece of approximately 30 mg (range 15.1 57.5) for stable isotope enrichments was wiped clean from blood in ice-cold saline, weighed, and snap frozen.
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On day 15, 6 pigs per treatment were euthanized to obtain intestinal tissue samples for later histological and gene expression analyses.
One section of each explant was immediately placed in RNA later RNA Stabilization Reagent from QIAGEN (for gene expression analyses), and stored overnight at 4°C.
Gonad tissue was sampled from the fish in a sterile and RNAse free manner and fixed immediately in RNA later® (Applied Biosystems, Foster City, CA, USA) for gene expression analyses and 10% phosphate buffered formalin for subsequent histological processing.
Gene expression analyses of the genes mentioned above were performed by quantitative PCR.
Gene expression analyses are important for the revealing of gene functions.
The results of gene expression analyses will be published at a later stage.
We combined these two approaches by performing global gene expression analyses in a test cohort to identify a panel of genes that later could be validated in an independent cohort.
Gene expression analyses by qRT-PCR revealed significant changes in expression of several glycolytic genes.
The mCherryHiCD271- population was sorted for gene expression analyses.
Data from gene expression analyses of fracture healing provide the basis for understanding these processes [15].
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