Cells were collected 24 h later and further experiments were conducted.
Three weeks later in vivo microdialysis experiments were conducted to measure basal and stimulus evoked overflow of DA from the striatum.
The males' antennae&maxillary palps were ablated (NAM) when the flies were one-day-old and the experiments were conducted seven days later.
The fear recall experiments were conducted a day later in the experimental arena described above, using the same procedure without the electrical foot shocks.
Cells were passaged after 12 hr onto poly-L-lysine coated glass coverslips in Hank's Buffered Saline Solution (HBSS: 140 mM NaCl, 4 mM KCl, 1 mM MgCl2, 1.8 mM CaCl2, 10 mM Hepes, 5 mM glucose at pH 7.4) and experiments were conducted 2 hr later.
Initially, experiments were conducted using 20 μg genomic DNA, later we reduced this to 10 μg.
These experiments were conducted as part of the controversial, top-secret CIA program later revealed as Project MKUltra.
Three independent experiments were conducted.
Experiments were conducted under approved Institutional Animal Care and Use Committee protocols.
All the experiments were conducted in triplicate.
