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Acidic treatment was performed one hour later by replacing the culture medium with DMEM acidified with citric acid (Sigma-Aldrich) at pH 5.0 for 15 min. Medium was further aspirated and replaced by complete medium.

The reaction was stopped later by replacing transfection media with the normal growth media.

The transfected cells were selected 48 h later by replacing the medium with one containing 800 μg/ml G-418 disulphate (Formedium).

When tested 24 hr later by replacing the mice in the same context, the misaligned mice exhibited a significant reduction in fear-conditioned behaviour (ZT 2, p < 0.0001; Figure 6A, left), indicating that circadian misalignment affects long term memory.

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Sampling was originally intended to include "normal" pigs selected by the farmer, but was later on replaced by selection performed by abattoir technicians.

The former was disbanded three years later and replaced by the Department of Energy.

Atkinson was sacked two days later and replaced by Aberdeen's Alex Ferguson.

Serra Ferrer was sacked a week later and replaced by Carles Rexach.

The 15th MEU would later be replaced by the 13th MEU as the last surge unit.

Therapy must start intravenously and can later be replaced by oral therapy depending on clinical and radiological responses.

The original method using iodine proved to be too unspecific and was later on replaced by the specific acetylacetone method.

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