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Further improved assays were introduced later and validated against an RT-PCR provided by Canada's National Microbiology Laboratory.
Using antibodies recognising PfG377 as the primary marker and α-tubulin II as a secondary marker, robust estimates of sex ratio in in vitro cultures were obtained for gametocytes at stage IV or later, and validated by light microscopic counts.
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It was later discussed and validated again in the entire research group before reaching a final consensus.
The use of elevated serum levels of type I interferon-regulated chemokines as biomarkers for disease activity was suggested in early studies [ 73] and was later replicated and validated by associations between elevated transcript levels of these chemokines with disease activity and organ damage [ 74, 75].
Later we developed and validated our own competitive real-time quantitative PCR as described elsewhere [57].
Focus group discussions were audio recorded and later transcribed verbatim and validated.
55 It has later been adjusted and validated for the evaluation of meniscal injuries.
The findings presented in this section were later universally acknowledged and validated by a multitude of healthcare providers, academics, policymakers and patients spoken to at a healthcare conference in Erbil.
With an average annotation accuracy of 83.9% for pollen semantics and 98.6% for spore semantics, the semantic models built for morphology annotation demonstrably produced good predictions based on grain morphology that can later be reviewed and validated by palynologists.
A numerical example was given and validated later by FEM analysis.
More advanced and later versions will be evaluated and validated in large scale pilots.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com