Clones were selected one day later, and sequenced.
Clones were selected one day later, and sequenced using the forward and reverse primers from upstream and downstream sequences of the antisense insertion.
The identity of most of algal samples was verified by polymerase chain reaction (PCR) amplification (discussed later) and sequencing of the internal transcribed spacer and/or small subunit (SSU) ribosomal DNA (rDNA) regions, and when necessary (in some green algae), phylogenetic analyses were conducted to confirm the assignment into specific algal classes (data not shown).
Later, gene duplication and sequence divergence occurred and selection of specific 5'–3' tRNA sequences would have increased the specificity of tRNAs along with the usage of various amino acids.
The sequences analyzed were derived from strains collected between year 1933 and 2012 (more than 90% later than 2000), and sequence data were obtained from the GISAID (Global Initiative on Sharing Avian Influenza Data) EpiFlu™ Database.
And lo and behold the studio agreed, and we reshot the sequence three weeks later and that's the sequence that's in the film.' Where, I ask Mendes, does that confidence come from?
The new "class A" is discussed later and includes the sequences of extracellular matrix protein 2 (ECM2), ECM2-like protein from the X chromosome (ECMX), ECM2-like proteined proteins upstream ofrome DCN gene in fish genomes (ECMZ), and the small leucine-rich repeat protein from Ciona intestinalis and Ciona savignyi (SLRP1) (see below).
During rehearsals, a number that involves intense pounding on a wooden floor led to splinters during a later barefoot sequence; and one dancer was kicked in the hand during a martial arts routine.
There is the "Sounds of Silence" montage and later the sequence in which Benjamin drives to the Robinson house to pick up Elaine for their second date, against Mrs. Robinson's wishes.
For εw∗ ca. −0.18, the partition coefficients equal (KH = KL) and later their sequence inverts.
