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Exact(13)
Genistein was added 8 hours later and cells incubated with compound for 16 hours.
The medium was removed 2 h later, and cells were lysed with 100 μl of DMSO.
Supernatants were removed 18 hours later and cells lysed for RNA isolation.
Infectious medium was changed 12 h later and cells were kept in culture until following analysis.
Transfection medium was discarded 10 h later and cells were washed twice using phenol red-free DMEM.
Culture medium was replaced with fresh medium 24 h later, and cells were irradiated with 5 Gy X-rays.
Similar(47)
Cells were harvested 24 hrs later and cell extracts were assayed for luciferase, β-galactosidase and renilla luciferases, as stated in Table 2.
Cells were lysed 24-36 h later, and cell extracts were used to measure firefly luciferase and β-galactosidase activities, respectively.
The aggregated cells would migrate up to the surface of the ridge later, and the cells on the ridge align with the direction of the ridge-groove patterns.
The spleen was removed five hours later, and CD11c+ cells (DCs) were purified by magnetic bead separation (Miltenyi Biotec).
The medium was changed 24 hr later, and the cells were allowed to grow to confluence.
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CEO of Professional Science Editing for Scientists @ prosciediting.com