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Another aliquot of 0.75 ml was collected and stored in the freezer at −20 °C for later analysis with the automated system.
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All behavioural tests were recorded with a Sony Handicam DCR-SX 83E for later analysis with Noldus Ethovision XT 7.0 (Noldus Information Technology, Wageningen, The Netherlands).
The calculated parameters were then extracted for later analysis with Microsoft Excel.
This association was confirmed in our later analysis with a larger study sample size (Yuan et al, 2004).
The other 1 ml solution was transferred to a PET bottle and stored at −20 °C for later analysis with ICP-MS (Cd, As, Cr, Mn, Ni, Pb, and Hg).
For the later analysis, the single strand RAA in the stems' tips were lumped with the REC.
A later analysis of the Leptocerinae, with more character data, included this larva but without explanation [ 19].
This same sequence, however, was also used in a later analysis by the same authors [ 67], with no ill effects.
The normalisation step was performed using invariant normalisation [ 50] to address the fact that the majority of genes change expression, with the later analysis stages performed with limma.
This limitation is overcome later in the analysis, with the t-tests as described in the methods section.
In addition, the two first years of data were used in the later analysis, which precludes most of the section with largest errors of the illustrated data.
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