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The genes composing a cluster selected at the early stage (day 3) were defined as an early responding gene-set and those composing a cluster selected at the late stage (day 5) were defined as a late responding gene-set.
These transcription factors further controls expression of adipocyte-specific genes (e.g. FAS, ACS1, FABP4, FATP1 and perilipin) at the late stage (day 5 post-confluence) leading to the fat droplet formation [ 64].
Cluster 1 contains genes with high expression at early differentiation stages (day 2 and day 4) and reduced expression at the late stage (day 7), for example KRT1 and KRT10, and is enriched with genes involved in epidermis development, blistering, and hyperkeratosis.
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By contrast, the mRNA and protein levels of C/EBPalpha in control cells were barely detectable at late stages (day 21) of differentiation.
Anthony was brilliant in the late stages Sunday, driving the Knicks to victory with 41 points, including a 12-point fourth quarter and a huge 3-pointer in the final minute.
This conclusion was drawn from the observation that most INS-GFP+ cells in late-stage (day 20 25) cultures produced other endocrine hormones, most commonly glucagon.
The persistence of an excess risk up to several years after stopping drinking indicates that alcohol is probably not only a late stage carcinogen (Day and Brown, 1980), as previously observed for oral, pharyngeal (Franceschi et al, 2000), and oesophageal (Bosetti et al, 2000) cancers.
To more directly study the role of Ubc9 in the cell cycle, we stained lymph glands in late third instar stage (day 6.5 7) for phospho-histone H3 (Fig. 4A F).
Thomas was only asked, in various ways, to address the exchange he had with fans at Madison Square Garden during the late stages of Monday's 99-89 loss to the Dallas Mavericks.
Secondary haustoria are established from day 2-3, and by the late stage of infection at day 6-7, very large numbers of haustoria are present in the host epidermal cells, as seen in Figure 1.
bFGF was included in cultures through the late EB to NE stage (day 5 16) to generate dorsopallial NE cells.
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