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The βGBP cytokine has previously been shown to negatively regulate the cell cycle by blocking cells in late S phase.
A vast amount of work over the years indicates that different origins along each eukaryotic chromosome are activated in early, middle or late S phase.
It phosphorylates histone H2B at tyrosine 37 in the nucleosomes found upstream of the histone gene cluster, and this suppresses histone transcription in late S phase.
Within the S phase, DT/pU6-m3 had about 1.5 times more cells in early than late S, while DT/siTR1 had about 3 times more cells in early than late S.
Based on timing and morphological criteria, these observations suggested that UNG2 forms foci during late S phase or early G2.
HRR represents high-fidelity DSB repair occurring mainly in late S and G2 of the cell cycle [20].
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Late S-phase stage nuclei [39] were visualized for clarity of replicating foci colocalization, or lack thereof.
S-phase was divided into early, mid and late S-phase based on total DAPI intensity.
It may be this dissociation of Rif2 in late S-phase that allows telomere elongation.
This was followed by immunofluorescence to score for cells in early, mid, and late S-phase of cell cycle.
However, this hypothesis relied on a single microarray experiment corresponding to only two time points in late S-phase.
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