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The late recruitment of the V-ATPase in 3d/3d phagosomes might suggest a defect in phago-lysosome fusion.
One particular feature of DCs is the slightly acidic pH of their cross-presentation compartments, which is generated by the late recruitment of the V-ATPase (the principal proton carrier in the lysosomes) that acidifies the lysosomes and the early recruitment of the NADPH oxidase 2 complex (NOX2), which slows down the acidification by consuming protons4.
These results suggest that the loss of OCRL impairs uncoating, a finding consistent with its very late recruitment at pits.
Note that their late recruitment is accepted by Soll and Ibba as well (see, for example, [ 64, 65]).
The late recruitment of RNAPII addresses the apparent contradiction that the parasite chromatin is closed in the late schizont stage despite the appearance of late-stage mRNAs.
Such comparisons reveal correlations between the observed segregations of species following amino acid usage, and the separation of amino acids following early or late recruitment.
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Furthermore, the amino acids for which detailed aptamer data is available are those that have complex side chains (which, presumably, mediate interactions with the aptamers) and are thought to be late recruitments to the genetic code [ 113].
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