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Lastly, sections were immersed in 0.02% toluidine blue in 0.25% glacial acetic acid, pH 3.2.
Lastly, sections were counterstained with hematoxylin and analyzed by standard light microscopy.
Lastly, sections were incubated with Biotinyl Tyramide working solution and streptavidin-HRP.
Lastly, sections were rinsed with water and treated with graduated-density alcohol and xylol, as previously described by Matsui et al. in 1998 [ 21].
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Lastly, section 102.166 7)(b) provides that, "if a counting team is unable to determine a voter's intent in casting a ballot, the ballot shall be presented to the county canvassing board for it to determine the voter's intent".
Lastly, Section 8 concludes.
Lastly, Section 7 presents the concluding remarks.
Lastly, Section 7 concludes the paper.
Lastly, Section 8 summarizes the paper.
Lastly, Section 6 draws the conclusions.
Lastly, Section 5 mentions further research developments.
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