Suggestions(2)
Exact(4)
The third or last round of selection is made after genotyping of BC3F2 or BC4F2 individuals.
To identify these sequences, DNA recovered from the last round of selection was cloned and sequenced.
The study did not characterize affinities and specificities of candidate ssDNA MREs from the last round of selection.
In the last round of selection preformed biotinylated xyloglucan-phage-complexes were caught on streptavidin coated paramagnetic beads in the presence or absence of soluble xyloglucan in an attempt to vary the degree of selection stringency further.
Similar(56)
Selected numbers of single colonies from the last round of positive selection were screened by replication onto plates with varied concentrations of chloramphenicol (34, 50, 75, 100, 150, or 250 μg/mL) in the presence and the absence of the appropriate unAA.
The cDNA coming from the last round of the selection procedure was PCR-amplified using Taq DNA-polymerase (Fermentas) with the primers P1 (5'-TAATACGACTCACTATAGGGTACGCTGAAACAG-3') and P2 (5'-CCTCCGAAACAGGACTGTCAGGGGGTACCAG-3') [ 21], and in vitro transcribed with T7 RNA-polymerase (Fermentas) over night at 37°C.
In addition, selection for ethanol tolerance after each round of shuffling yielded F3 hybrids that show a higher ethanol production than those obtained from crosses where no selection was applied until after the last round of genome shuffling, indicating that selection for ethanol tolerance can in some cases increase the chances of selecting hybrids with increased ethanol production.
Moreover, for these two strategies, we compared the effects of selecting a subpopulation of superior hybrids in terms of ethanol tolerance after each round of shuffling, versus a parallel experiment in which we did not apply any selection until after the last round of shuffling (see further).
But a selection of them return here to answer one last round of classic dating questions posed by readers through the ages.
This is the last round of question.
Top with last round of meringue.
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