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Then, after Mr. Klein made further tweaks to the design of the house, a third architect, Fred Stelle, was brought in to make the last refinements to the concept and execute the construction.
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In rock, though, the last refinement is feigning indifference, and it's not easy to imagine this group doing that now.
Finally, the results show the positive impact of the last refinement on reducing the complexity of the inference mechanism.
The lower section of Figure 5 shows part of the SSC obtained from the last refinement step.
An elongated electron density clearly visible in the Fourier difference map during the last refinement cycles was modelled using coordinates for polyethylene glycol (PEG 4000) from the HIC-Up database [54].
There were no correlations larger than 0.7 in the last refinement cycle.
For adaptive refinement, Fig. 5.5 provides a histogram of the knots in [ a, b ] of the last refinement step.
In the last refinement step an angle of 0.9° between consecutive reference reprojections was used, yielding 2134 reference images.
For adaptive refinement, we plot in Fig. 5.7 a histogram of the knots in [ a, b ] = [ 0, 1 ] of the last refinement step.
In the last refinement cycle, data up to a spatial frequency of 1/3.7 Å−1 was included for the refinement and 39,549 particles were used for the final reconstruction.
To do so, hydrogens were added to all atoms of the model from the last refinement, and followed by regularization and removal of hydrogens.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com