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The last pellets were resxuspended thoroughly by 50 μl of ddH2O followed by 1,000 g of centrifugation at 4°C for 1 min.
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We collapsed into our sleeping tents that night with the most amazing sense of relief that we'd got the last pellet out of the last baiting pod".
The last pellet is resuspended in 1/10th of the initial volume of KK2 and an aliquot is diluted to measure the OD650.
She desperately tried not to shit, becoming toxically constipated, but eventually her bowels would explode in protest, so she would scoop it up and smear it over her body trying to preserve every last pellet of her husband.
The last pellet was solubilized by boiling into 2x sample buffer (2% SDS, 10% glycerol, 100 mM DTT, 60 mM Tris-HCl [pH 6.8] and 0.001% bromophenol blue), and used for SDS-PAGE as described under Western blotting.
The last pellet consisted of mitochondrial fraction.
When you choose to stop feeding your fire, the last pellet will burn itself out into a glowing ember.
But remember make sure that every last pellet has been consumed before adding more food or you will pollute the tank.
The last colorless pellet was then rinsed twice with 50 μL cold Tris HCl (pH 7.4) and poured into a cryotube.
A day would last forever, Making pellets of bread, Waiting for a branch On a bare tree to move.
A day would last forever, Making pellets… Minister of our coming doom, preaching On the car radio, how right Your Hell and damnation sound to me As I travel these small, bleak… Everything about you, my life, is both make-believe and real.
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