Sentence examples for last elongation from inspiring English sources

In particular, our measurements suggest that in a passive extraocular muscle the force does not depend on the entire length history, but to a great extent is only a function of the last elongation to which it has been subjected.

We have recently shown that in monkey passive extraocular muscles the force induced by a stretch does not depend on the entire length history, but to a great extent is only a function of the last elongation applied.

Note that in physiologic conditions sequences of eye movements are always separated by at least 100 ms. In this sense, it is then tempting to conclude that passive extraocular muscles have essentially no memory: the force they generate is only a function of the last elongation to which they have been subjected, and it does not depend on the previous history.

An initial denaturation at 94°C for 9 min, was followed by 38 cycles at 94°C for 1 min, 60°C for 2.5 min (for primers 20/1607 and 1268/3039, and 3 min at 56°C for primers 3207/3457 and 3335/3952 72°CC for 2 min followed by one cycle were the last elongation was for 10 min. PCR products were sequenced on both strands using internal primers essentially as described [47].

The last elongation step lasted 5 minutes.

The last elongation step was extended to 7 min at 72°C.

After the last cycle, a final elongation at 72°C for 30 min took place and the amplification ended at 12°C.

Amplification conditions were as follows: 1 hold of 75°C for 3 min, 1 hold of 94°C for 2 min and 34 cycles of 94°C for 1 min, 49°C for 1 min and 72°C for 2 min. Final elongation step lasted for 10 min at 72°C.

The program used involved a denaturation step at 98 °C for 30 s, followed by 35 cycles of denaturation at 98 °C for 10 s, annealing at 52 °C for 30 s and elongation at 72 °C for 3 min, and a final elongation step at 72 °C for 10 min. Amplification products were checked on an agarose gel and sequenced with Oend by MilleGen (http://www.millegen.com/, last accessed April 30 , 2013.

Cycling parameters were as follows: 94°C for 5 min, followed by 30 cycles of 94°C for 0.5 min, 63°C for 0.5 min, and 72°C for 1 min, and at last an elongation step of 72°C for 10 min.

The stereocilia near the kinocilium start to elongate first and they finish elongation last, forming the tallest and the most lateral row.