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The amount of double stranded DNA in each PCR 2 product was then measured using Quant-iT PicoGreen on a Fusion αHT Packard BioScience Company laser plate reader with the following settings: intensity = 5, time per row = 0.1, read per well = 1, high intensity (due to the expected high amounts of DNA).
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Other methods include powder rolling, laser plating and forming high melting point alloy (Qaim Syed, 2011; Stolarz et al., 2015); 4.
Other methods include powder rolling, laser plating and forming high melting point alloy (Qaim Syed, 2011; Stolarz et al., 2015); Solid state targets require more manual handling which causes a higher radiation hazard for personnel, especially when the target must be retrieved shortly after irradiation.
Typing something with letters on opposite ends of the keyboard — like "laser" or "plate" or "mail" or a billion other words — becomes a pretty hilarious thumb stretching exercise.
The microbiopsy devices used in our studies were fabricated by laser cutting plates of stainless steel.
The particles were ignited using a CO2 laser heated hot plate at 670 K, whereas the pellets were ignited using an electrical spark.
Direct measurement of kinematic error requires special devices such as laser interferometers, grid plate encoders or double ball bars, which impose production stop and specialized staff.
A cross-section of the Ti-6Al-4V lashockhock peened plate was electrochemically polished to avoid induced residual stress modification due to grinding.
One micro liter samples were spotted onto a matrix assisted laser desorption/ionization (MALDI) plate.
We pipeted 0.5 µL of this mixture onto each spot of a 192-spot matrix-assisted laser desorption/ionization (MALDI) plate (Applied Biosystems, Framingham, MA).
To minimize experimental error, we tested variable parameters, including focus mass, laser intensity, target plate and data-acquisition time.
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