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Tumour cells were selectively microdissected using a laser microdissection device (ION LMD, Jung Woo International, Co, Seoul, Korea).
Tissue samples were microdissected using an LMD6000 laser microdissection system (Leica Laser Microdissection System; Leica Microsystems, Wetzlar, Germany).
Slides were microdissected immediately following staining using a µCut Laser Microdissection system (Nikon Eclipse TE200).
However, the male breast cancer cases were microdissected by a scalpel and the female breast cancer cases by laser microdissection.
Laser microdissection.
The tumor and peripheral non-tumor cells in these FFPE tissues were collected with laser microdissection (LMD).
In this study, a W chromosome library, which had been constructed by laser microdissection of W chromosomes, was screened.
Laser microdissection permits the isolation of single cells from a heterogeneous tissue [25].
The laser microdissection technology allows for enrichment of specific cell types.
By laser microdissection cancer cell populations were harvested and subjected to whole genome expression analyses.
These results confirm the feasibility and the specificity of our laser microdissection based procedure.
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