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The ejector handles a motive flow of nearly 120 g s−1 and an entrained laser flow of nearly 3 g s−1.
Samples were immediately stained with fluorescent dyes using the BacLight kit (Molecular Probes Inc., Eugene, OR) and analyzed with a Coulter XL-MCL single laser flow cytometer.
Probably, the actual role of appropriate tube size is to maintain homogenous gas and straight laser flow towards the target material to generate plume rapidly without lifted off and simultaneously decreases target pitting.
RNase cocktail (250 U; RNase A, RNase T1) was added and the parasites incubated in the dark at room temperature for 30 min. Nuclear DNA content was measured based on fluorescence (FL-1) using a 488 nm argon laser flow cytometer.
DNA quantities were measured using a FACScalibur laser flow cytometer (Beckton Dickinson, USA) with Cellquest Software.
Stained samples were analysed on the FACS Calibur™ (BD) Neon-Argon dual laser flow cytometer.
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Flow cytometry was performed on a FACScan laser flow-cytometry system (Becton Dickinson, San Jose, CA, USA), and data were analyzed with the CellQuest software (BD Bioscience, San Jose, CA, USA).
Data from the reaction were acquired using the Bio-Plex suspension array system, a dual-laser, flow cytometry-based microplate reader system (Bio-Rad, Hercules, CA).
All samples were analyzed on the three-laser flow cytometer (LSR II, BD Biosciences) at the Flow Cytometry Core of the University Kansas Medical Center.
Cell acquisition was performed with a dual-laser flow cytometer (LSR-II, BD Biosciences, Mountain View, CA ) and the data were analyzed using FlowJo software (Tree Star, INC., Ashland, OR).
All antibodies were purchased from BD Bioscience, San Diego, CA. Cells were stained for 10 min at room temperature, washed in phosphate buffered saline with 1% bovine serum albumin and 0.1% sodium azide, then fixed in 1% formaldehyde and analyzed using a dual-laser flow cytometer (FACSCaliber; Becton Dickinson, San Jose, CA) and CellQuest software (BD Bioscience).
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