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Cell−backpack complexes were analyzed using a Horiba LA-950 V2 laser diffraction system.
For accurate, time-dependent droplet size distribution analysis, a laser diffraction system (Spraytec, Malvern, UK) was used.
A laser diffraction system was used to obtain accurate, time-dependent, quantitative measurements of the size and number of droplets expelled by the cough aerosol.
The emphasis of this study was on the development and refinement of a cough aerosol model in healthy human volunteers, detected and verified with a laser diffraction system.
While testing and tuning the laser diffraction system we found that our initial concerns were inaccurate: Healthy non-smokers are excellent models to characterize cough aerosol droplets.
The first limitation of this study was that the laser diffraction system, according to the manufacturer, was not intended to assess aerosolized mucus from the airways when coughing.
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Particle sizing was accomplished on a Microtrac s3500 laser diffraction analyzer (Microtrac Systems).
Particle size distributions were determined using the Horiba Partica LA-950 V2 laser diffraction particle size analysis system.
To achieve this we required a fast acquisition and high-resolution system, the laser diffraction technique, to capture the cough droplet size and number distribution to overcome the limitation of velocity and evaporation.
The particle size and drug-loading rate of all the samples were determined by a laser diffraction particle size analyzer and high performance liquid chromatography (HPLC) system.
The grains that were finer than 3φ were classified by a laser diffraction particle-size analyzer under wet dispersion conditions, using the Sympatec HELOS & RODOS systems (Clausthal-Zellerfeld, Germany).
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