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Images of stained larvae oriented in lateral, dorsal, and ventral views were obtained using an inverted confocal microscope (Zeiss, Cambridge, UK), with a 10× objective lens.
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The larvae were oriented in an upright position, illuminated with fiber optic lights and viewed through a dissecting microscope positioned over the drum, which rotated at 5 rpm clockwise and then counter clockwise, for 30 seconds in each direction.
All embryonic stages and the hatching larva are oriented with the anterior to the top.
For proper image processing, the larvae ideally have to be oriented in a lateral position.
Compared with other spiralian larvae, the direction of moving is shifted by 90 degrees relative to the position of the apical organ and prototroch: while the "typical" trochophore is orientated with the apical organ forward, the larva of Loxosomella murmanica is oriented with the frontal organ in front during its alternating swimming and creeping movements.
Whereas a larva may be facing any direction as it leaps, wheeling requires it to be oriented along the axis of the wind.
In ascidian larvae, swimming activity consists of three distinct patterns: oriented tail flicks, regular spontaneous swimming and photosensitive swimming.
"We're goddess oriented.
It's singles oriented, not artist oriented.
"This is family oriented.
His fealty was financially oriented.
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