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Contrary to the expectations, the maximum larvae mass estimated with the von Bertalanffy curve, increased by the pollution gradient.
When comparing the average larvae mass, we also included the size of the brood as an explanatory variable as brood size strongly predicts the average larvae mass at dispersal (Schrader et al., 2015).
The relationship suggests that the higher the M. sexta larvae mass, the lower the seed capsule number production per plant.
To analyse the success of the brood, we included as response variables the size of the brood, and the average larvae mass (variable obtained by dividing the total brood mass by the brood size).
A Levene's test for homogeneity of variance showed that the variance in the 'mite' and 'mite-free' treatment was the same for the brood size (F1,60 = 0.24; P = 0.62), and for the average larvae mass (F1,60 = 0.12; P = 0.72).
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But my next concern was, "What will happen in the next few years to the fish and shrimp eggs and larvae?" The mass die-off of newborn dolphins in the Gulf of Mexico in the winter of 2011 seemed an ominous reminder that adult survival does not guarantee good reproduction.
Feeding was allowed for 7 days, and the masses of the leaves consumed by the larvae and masses of the larvae were recorded for every other day for 7 days.
Larvae were mass-reared in groups of at least 50 and transferred as prepupae to sand as a pupation substrate.
For example, Ms larvae increased their mass by 1.5-fold when they fed on N-silenced plants in comparison to WT plants while another generalist larvae (Spodoptera exigua) increased their mass by 4-fold compared to WT-fed larvae [50].
WT plants infected with PST DC3000 showed enhanced resistance to PST DC3000 and decreased leaf consumption by M. sexta larvae, but larval mass gain was unaffected.
Secondly, using a Clark oxygen electrode, in which larvae to a mass of 50 mg (∼30 40 larvae) were placed in the recording chamber filled with 3 ml HL3 saline.
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