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Largest scaffolds of melon genome assembly v3.5.
Most of the carbohydrate- active enzymes [ 15] were distributed across the ten largest scaffolds of the P. carnosa genome sequence.
DNA of a single individual was deep-sequenced, and microsatellite markers were identified in 177 of the largest scaffolds of the sequence assembly.
Sequences of the 177 mapped largest scaffolds of the white grouper were BLASTN searched against Nile tilapia (Oreochromis niloticus) sequences in the Broad anchored database (v1.1) (http://cichlid.umd.edu/blast/blast.html).html
BLASTN search of the 177 largest scaffolds of white grouper against the Nile tilapia genome sequence identified strong similarity to 169 tilapia LG-anchored sequences and to 11 sequences of unknown location (UNK).
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The largest scaffold of the subset is 21.8 Mbp compared to the original 12.7 Mbp.
The hybrid assembly (Additional file 1 Figure S6) contains 56 scaffolds totaled 6,385,925 bp with N50 scaffold size of 213,399 bp and largest scaffold of 699,613 bp.
Along with the paired-end reads of Illumina sequences, these BAC end sequences allowed assembly of 193 contigs into 61 scaffolds with the largest scaffold of 684,936 bp.
A total of 10 additional miss-assemblies were identified by GAGE after scaffolding with the unamplified long-insert library (Table 4) and, comparably, the Phi amplified library created the same number of miss-assemblies but actually had the largest corrected N50, as well as containing the largest scaffold of all the assemblies.
This merged the two largest Nasuia-ALF contigs, leaving two large scaffolds of ∼102 kb and ∼12 kb in length.
In this study, whole genome comparisons including the new assembly of M. anisopliae, in which the largest scaffolds are of a comparable level of contiguity with those of M. robertsii, indicate a generally macrosyntenic conservation pattern.
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