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In the six libraries, miR165a showed the highest sequencing reads, which may suggest the largest expression in pear fruit among miRNA families.
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However, of all the differentially expressed genes, ependymin was a clear candidate and stood out as having the largest difference in expression levels between hierarchical levels, with ∼2-fold higher levels in sub-dominant than dominant, a 6.6-fold difference between sub-dominant and subordinate, and a 5.5-fold larger expression in the subordinate compared to the dominant.
qPCR revealing ubiquitous B4GAT1 and LARGE expression in all tissues analyzed, with highest expression of LARGE in brain and heart.
In this data set, parent node mutations resulted in large expression changes in their direct targets.
Genes with the largest expression fold change are shown in Supplementary Table 10.
To our knowledge, this is the largest expression study of CT genes in bladder cancer.
The genes have large expression ratios in the OneRA that are reduced in the TwoRA.
Conversely, in situ hybridization for Ihh and Col10a1 reveals larger expression domains in Rbpjk mutant/ Sox9 haploinsufficient embryos, and a wide domain of Mmp13 expressing cells.
Our results suggest that ratios are most commonly reduced among the genes with large expression ratios in the OneRA, thus likely to be differentially expressed.
Other technologies, including RNA sequencing, are also generating large expression datasets in multiple species.
The WEE1 gene harbours a large expression level in the pCR group.
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