Exact(4)
In case of the largest construct (cL2-13), no positive clone was identified.
The expansion, for example, of the largest construct made in this study (cL2-13, level 2-2, 33 kb) would require its reconstruction, but with an end-linker that adds two Esp3I restriction sites to the construct (end-linker pELR-4, Fig. 5).
Thus, using our retrofitting approach a success rate of 89% (8 out of 9) was obtained, with the largest construct spanning >213 kb (Table 1).
For example, our largest construct (FG-N-FSFG-K-tet) contains two fragments from Nsp1 (FG-N, turquoise; FSFG-K, green; full-length Nsp1 also shown with residue numbering), a separator (white) and the tetramerization domain of p53 (yellow).
Similar(56)
Much of the filming was carried out in a self-generating wave tank on a set in Taiwan, the largest constructed for a movie.
Its 1.2 million m2 grout curtain surface area is the largest constructed in the world to date.
It covered 25 ha, making it the largest constructed in Britain during the 70s.
I've been, myself, always in action; but I've been interested in its intellectual antecedents and the larger construct within which that action takes place.
However, such expansions of the construct risk crossing into instructional quality (Scheerens 2016, p. 20), in itself a large construct.
The optimal number of constituents in each new (larger) construct decreases as the size and complexity of the construct increase.
We also want to express our appreciation to Pamela Stanley for providing us the mouse Large construct.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com