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Northern analysis of sCAC381 revealed two bands (~300 bp and ~174 bp), indicating possible RNA processing or two transcriptional start sites (TSS for the larger transcript may be located upstream of the regular TSS, but this needs to verified using either strand specific sequencing or 5'RACE).
A larger transcript version of the second interview is available here via @exiledsurfer/mathewi.
This viral fusion transcript could be the additional larger transcript seen in the northern analysis of 27-001 in Figure 3c.
In the affected calf, there was, however, also an additional larger transcript of 5.8 kb present in all investigated tissues (Figure 3A).
The larger transcript could be a pre-mRNA of Prdm14 or the result of altered splicing due to retroviral integration at Evi32.
PS genes also tend to be much longer on average than MS genes, with a larger transcript length (73.3 Kb vs. 31.8 Kb) and a larger number of exons (18.9 vs. 5.5, see Table 1).
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Many rRNA molecules are cleaved from much larger transcripts and may also be methylated or enzymatically modified.
The chapter also discusses the important features of shRNAs and the use of a variety of promoters, including those that express shRNAs as part of larger transcripts, which is referred to as hybrid RNAi molecules.
Polymerase chain reaction products were also observed using cDNA from steroidogenic tissues and primers designed to regions specific for cStAR2, indicating that cStAR2 is expressed in tissues and may account for the presence of larger transcripts observed on Northern blots.
As first exons of transcripts, DAlFEs were predicted to have no splicing to upstream exons and no participation as alternatively spliced exons of larger transcripts.
The novel clones isolated here were cDNA fragments presumably derived from larger transcripts.
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