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RNA-seq has been shown to be quantitatively accurate over a larger range of expression levels than previous methods, such as microarrays [ 20– 20].
RNA-seq has been shown to be quantitatively accurate over a larger range of expression levels than previous methods, such as microarrays, while also being more effective at identifying genes that show low expression levels [ 1- 7].
It is expected that RNA-Seq methodologies will supersede microarrays for transcript profiling because of higher sensitivity, base-pair resolution and the larger range of expression values that can be detected [ 3, 5, 9].
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Slight differences in the developmental timecourses of neurons (visible only at early developmental stages) would certainly exacerbate the cell-to-cell variability in expression of ion channels already observed in mature neurons, and would reveal even larger ranges of expression of ion channels in immature neurons.
And the comparison, much to my shame, showed me that it was not the elegant pen marks and brief blocks of type that added the largest range of expression to a sentence.
There was a large range of expression with relative protein levels ranging from 0.06 to 2.4 for Mre11.
The library generated a large range of expression strengths and logic gating from simple re-positioning of the operator sequences of a two-input promoter.
The addition of some other monoses can 'turn down' the inducing activity in C. cellulolyticum, which may benefit the large range of expression regulation.
The Gateway® system facilitates the transfer of ORFs into a large range of expression vectors that are suitable for downstream studies.
The relaxed recognition by TBPs in TATAΔ-containing promoters might be responsible for a bias towards the large range of expression.
Since free Trp concentrations can be fairly low and still maintain highly charged tRNATrp, trpR-mediated repression acts over a large range of expression.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com