It is possible that part of the large FRET changes at pHext 7.5 resulted from a higher sensitivity of the FRET reporter in these conditions, as the shift in pHext may have changed the pHi.
The linker design was optimized to produce a large FRET effect.
This in turn drives the annealing of the complementary oligonucleotides which brings the fluorescence probes to close proximity producing large FRET signals proportional to the amount of target cells.
GFPndΔ-Epac-mRFP showed the largest FRET change but mRFP photobleaches more easily than the other two [8].
The second-best construct, GFPnd-Epac-tdTomato, shows an almost equally large FRET span and it is very resistant to photobleaching.
We considered two possible explanations for the large FRET fluctuations observed upon docking of dsDNA to the RecA filament.
The choice of the Cbx7 chromodomain was later revealed to be fortuitous since this binding domain produced the largest FRET ratio change of all domains tested.
The problem of "entitlement imbalances is so large," they fret, "that the regular political process seems unlikely to produce a solution," so they recommend a bipartisan "special process" insulated from popular pressures.
"Social Security Bashing: A Historical Perspective" (Economic View, Jan . 16 said that before Social Security was enacted, "executives of the nation's largest corporations fretted that Americans would abandon thrift if the government began to guarantee retirement incomes".
The other side of the room is a brick wall punctuated by large windows fretted with a geometric wood design.
